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citrate buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher citrate buffer
    Citrate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+a/Citrate+Buffer/10__22203_slash_ecm__v033a11-128-23-28
    Average 99 stars, based on 1 article reviews
    citrate buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Ifn1 is an intracellular GMP 5'-nucleotidase induced during the fission yeast response to phosphate starvation.
    Article Snippet: .. Cells were thawed and resuspended in 25 mL of buffer A (50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 20 mM imidazole, 1 mM DTT, and 20% glycerol) containing 1 mg/mL lysozyme and 1/2 tablet of EDTA-free protease inhibitor cocktail (ThermoFisher). ..

    Article Title: Ifn1 is an intracellular GMP 5′-nucleotidase induced during the fission yeast response to phosphate starvation
    Article Snippet: .. Cells were thawed and resuspended in 25 mL of buffer A (50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 20 mM imidazole, 1 mM DTT, and 20% glycerol) containing 1 mg/mL lysozyme and 1/2 tablet of EDTA-free protease inhibitor cocktail (ThermoFisher). ..

    Fractionation:

    Article Title: CPF-CF-terminated snoRNAs shuttle through the cytoplasm via an mRNA guard protein-mediated surveillance mechanism
    Article Snippet: Cells were lysed in PBSKMT buffer (1x PBS, pH 7.4, 3 mM KCl, 2.5 mM MgCl 2 , 0.5% (v/v) Triton X-100, protease inhibitor (Roche)) using the FastPrep ® -24 machine (MP Biomedicals) three times for 30 s at 5.5 m/s. .. For the cytoplasmic fractionation, the cells were lysed in 500 μl lysis buffer (18 % Ficoll 400, 10 mM HEPES pH 6.0), 1 ml buffer A (50 mM NaCl, 1 mM MgCl 2 , 10 mM HEPES pH 6.0), and 1 μl Ribolock RNase Inhibitor (Thermo Fisher Scientific), followed by vortexing and centrifugation to obtain the supernatant as the cytoplasmic fraction. ..

    Article Title: CPF-CF-terminated snoRNAs shuttle through the cytoplasm via an mRNA guard protein-mediated surveillance mechanism.
    Article Snippet: Cells were lysed in PBSKMTbuffer (1x PBS, pH 7.4, 3mMKCl, 2.5mMMgCl2, 0.5% (v/v) Triton X-100, protease inhibitor (Roche)) using the FastPrep®-24 machine (MP Biomedicals) three times for 30 s at 5.5m/s. .. For the cytoplasmic fractionation, the cells were lysed in 500 μl lysis buffer (18 % Ficoll 400, 10mM HEPES pH 6.0), 1ml buffer A (50mM NaCl, 1mM MgCl2, 10mM HEPES pH 6.0), and 1 μl Ribolock RNase Inhibitor (ThermoFisher Scientific), followedby vortexing and centrifugation to obtain the supernatant as the cytoplasmic fraction. ..

    Lysis:

    Article Title: CPF-CF-terminated snoRNAs shuttle through the cytoplasm via an mRNA guard protein-mediated surveillance mechanism
    Article Snippet: Cells were lysed in PBSKMT buffer (1x PBS, pH 7.4, 3 mM KCl, 2.5 mM MgCl 2 , 0.5% (v/v) Triton X-100, protease inhibitor (Roche)) using the FastPrep ® -24 machine (MP Biomedicals) three times for 30 s at 5.5 m/s. .. For the cytoplasmic fractionation, the cells were lysed in 500 μl lysis buffer (18 % Ficoll 400, 10 mM HEPES pH 6.0), 1 ml buffer A (50 mM NaCl, 1 mM MgCl 2 , 10 mM HEPES pH 6.0), and 1 μl Ribolock RNase Inhibitor (Thermo Fisher Scientific), followed by vortexing and centrifugation to obtain the supernatant as the cytoplasmic fraction. ..

    Article Title: CPF-CF-terminated snoRNAs shuttle through the cytoplasm via an mRNA guard protein-mediated surveillance mechanism.
    Article Snippet: Cells were lysed in PBSKMTbuffer (1x PBS, pH 7.4, 3mMKCl, 2.5mMMgCl2, 0.5% (v/v) Triton X-100, protease inhibitor (Roche)) using the FastPrep®-24 machine (MP Biomedicals) three times for 30 s at 5.5m/s. .. For the cytoplasmic fractionation, the cells were lysed in 500 μl lysis buffer (18 % Ficoll 400, 10mM HEPES pH 6.0), 1ml buffer A (50mM NaCl, 1mM MgCl2, 10mM HEPES pH 6.0), and 1 μl Ribolock RNase Inhibitor (ThermoFisher Scientific), followedby vortexing and centrifugation to obtain the supernatant as the cytoplasmic fraction. ..

    Centrifugation:

    Article Title: CPF-CF-terminated snoRNAs shuttle through the cytoplasm via an mRNA guard protein-mediated surveillance mechanism
    Article Snippet: Cells were lysed in PBSKMT buffer (1x PBS, pH 7.4, 3 mM KCl, 2.5 mM MgCl 2 , 0.5% (v/v) Triton X-100, protease inhibitor (Roche)) using the FastPrep ® -24 machine (MP Biomedicals) three times for 30 s at 5.5 m/s. .. For the cytoplasmic fractionation, the cells were lysed in 500 μl lysis buffer (18 % Ficoll 400, 10 mM HEPES pH 6.0), 1 ml buffer A (50 mM NaCl, 1 mM MgCl 2 , 10 mM HEPES pH 6.0), and 1 μl Ribolock RNase Inhibitor (Thermo Fisher Scientific), followed by vortexing and centrifugation to obtain the supernatant as the cytoplasmic fraction. ..

    Article Title: CPF-CF-terminated snoRNAs shuttle through the cytoplasm via an mRNA guard protein-mediated surveillance mechanism.
    Article Snippet: Cells were lysed in PBSKMTbuffer (1x PBS, pH 7.4, 3mMKCl, 2.5mMMgCl2, 0.5% (v/v) Triton X-100, protease inhibitor (Roche)) using the FastPrep®-24 machine (MP Biomedicals) three times for 30 s at 5.5m/s. .. For the cytoplasmic fractionation, the cells were lysed in 500 μl lysis buffer (18 % Ficoll 400, 10mM HEPES pH 6.0), 1ml buffer A (50mM NaCl, 1mM MgCl2, 10mM HEPES pH 6.0), and 1 μl Ribolock RNase Inhibitor (ThermoFisher Scientific), followedby vortexing and centrifugation to obtain the supernatant as the cytoplasmic fraction. ..

    Article Title: Structural Evolution of LEAFY Reveals DNA-Mediated Cooperativity and Dimerization Shifts at the Water-to-Land Transition
    Article Snippet: Oligonucleotides and details on the cloning strategy are provided in The pETM-11-based expression vectors were introduced in Escherichia coli BL21 cells strain (Novagen). .. Cell cultures were grown at 37 °C to reach OD 600 of 0.6, then the cultures were cooled at 18 °C and protein expression was induced by adding 1 mM of IPTG for 12 h. Cultured cells were collected by centrifugation and the pellet was resuspended in Buffer A (Tris-HCL 20 mM pH 8, TCEP 1 mM) supplied with complete protease inhibitors (Thermo). ..

    Article Title: Type VIIb secretion system recruits the dedicated cell wall hydrolase EssH to enable effector secretion by Staphylococcus aureus .
    Article Snippet: The blots were developed on Amersham Hyperfilm ECL (GE Healthcare Life Sciences) or using ChemiDoc imaging system (Bio-Rad). .. Bacteria were cultured under T7 permissive conditions to OD600~3.0, harvested by centrifugation for 10 min at 8,000 × g at 4°C and suspended in Buffer A (20 mM Tris pH 8, 300 mM NaCl, 10% vol/vol glycerol, Pierce EDTA-free protease inhibitors). ..

    Expressing:

    Article Title: Structural Evolution of LEAFY Reveals DNA-Mediated Cooperativity and Dimerization Shifts at the Water-to-Land Transition
    Article Snippet: Oligonucleotides and details on the cloning strategy are provided in The pETM-11-based expression vectors were introduced in Escherichia coli BL21 cells strain (Novagen). .. Cell cultures were grown at 37 °C to reach OD 600 of 0.6, then the cultures were cooled at 18 °C and protein expression was induced by adding 1 mM of IPTG for 12 h. Cultured cells were collected by centrifugation and the pellet was resuspended in Buffer A (Tris-HCL 20 mM pH 8, TCEP 1 mM) supplied with complete protease inhibitors (Thermo). ..

    Cell Culture:

    Article Title: Structural Evolution of LEAFY Reveals DNA-Mediated Cooperativity and Dimerization Shifts at the Water-to-Land Transition
    Article Snippet: Oligonucleotides and details on the cloning strategy are provided in The pETM-11-based expression vectors were introduced in Escherichia coli BL21 cells strain (Novagen). .. Cell cultures were grown at 37 °C to reach OD 600 of 0.6, then the cultures were cooled at 18 °C and protein expression was induced by adding 1 mM of IPTG for 12 h. Cultured cells were collected by centrifugation and the pellet was resuspended in Buffer A (Tris-HCL 20 mM pH 8, TCEP 1 mM) supplied with complete protease inhibitors (Thermo). ..

    Article Title: Type VIIb secretion system recruits the dedicated cell wall hydrolase EssH to enable effector secretion by Staphylococcus aureus .
    Article Snippet: The blots were developed on Amersham Hyperfilm ECL (GE Healthcare Life Sciences) or using ChemiDoc imaging system (Bio-Rad). .. Bacteria were cultured under T7 permissive conditions to OD600~3.0, harvested by centrifugation for 10 min at 8,000 × g at 4°C and suspended in Buffer A (20 mM Tris pH 8, 300 mM NaCl, 10% vol/vol glycerol, Pierce EDTA-free protease inhibitors). ..

    Bacteria:

    Article Title: Type VIIb secretion system recruits the dedicated cell wall hydrolase EssH to enable effector secretion by Staphylococcus aureus .
    Article Snippet: The blots were developed on Amersham Hyperfilm ECL (GE Healthcare Life Sciences) or using ChemiDoc imaging system (Bio-Rad). .. Bacteria were cultured under T7 permissive conditions to OD600~3.0, harvested by centrifugation for 10 min at 8,000 × g at 4°C and suspended in Buffer A (20 mM Tris pH 8, 300 mM NaCl, 10% vol/vol glycerol, Pierce EDTA-free protease inhibitors). ..



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